NASC Stock Detail Page
NASC ID: N30917
Name: NFB1
ABRC stock number: CS30917
Description: homologous to human CAF1 subunit B or yeast CAC2, the p60 subunit s of human and yeast chromatin assembly factor I , respectively. Human CAF1 is composed of three subunits that are 150 kd, 60 kd and 48 kd in size. The smallest subunit dissociates during G1 phase of the cell cycle. CAF1 is thought to deposit histones H3 and H4 onto newly replicated DNA such that histone H2A and H2B can then bind to form nucleosome core particles.
Donation Date: 2002-11-27
Donated by: | University of Arizona Natalie Doetsch |
Donor Number: T-FGC446B
Part of : CFGC RNAi silencing
Stock type: individual line
Material type: seed
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Seed type: RNA silencing
Mutagen: ema
Background: Ws-2(N2360)
Marker used: T-DNA marker=Hygromycin
Transformation method: This mutant was produced using Agrobacterium-mediated transformation using a binary vector containing a fragment of the target gene in an inverted repeat orientation for RNAi silencing. The transformation was carried out using the floral dip method. The transgene is a single-copy or low-copy insertion event, as determined by DNA gel blot analysis (data will be posted at ChromDB: (http://chromdb.org/).
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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Phenotype
homologous to human CAF1 subunit B or yeast CAC2, the p60 subunit s of human and yeast chromatin assembly factor I , respectively. Human CAF1 is composed of three subunits that are 150 kd, 60 kd and 48 kd in size. The smallest subunit dissociates during G1 phase of the cell cycle. CAF1 is thought to deposit histones H3 and H4 onto newly replicated DNA such that histone H2A and H2B can then bind to form nucleosome core particles.